Engineered protein coatings to improve the osseointegration of dental and orthopaedic implants.

摘要: Here we present the design of an engineered, elastin-like protein (ELP) that is chemically modified to enable stable coatings on surfaces titanium-based dental and orthopaedic implants by novel photocrosslinking solution processing steps. The ELP includes extended RGD sequence confer bio-signaling for mechanical stability. thin films were fabricated cp-Ti Ti6Al4V using scalable spin dip coating processes with photoactive covalent crosslinking through a carbene insertion mechanism. withstood procedures mimicking screw hip replacement stem implantations, key metric clinical translation. They promoted rapid adhesion MG63 osteoblast-like cells, over 80% after 24 h, compared 38% uncoated Ti6Al4V. cells produced significantly more mineralization Human bone marrow mesenchymal (hMSCs) had earlier increase in alkaline phosphatase activity, indicating osteogenic differentiation mineral deposition adhesive coatings. Rat tibia femur vivo studies demonstrated cell-adhesive ELP-coated increased bone-implant contact area interfacial strength one week. These results suggest withstand surgical implantation promote osseointegration, enabling implant loading potentially preventing micromotion leads aseptic loosening premature failure.