Role of Water in the Specific Binding of Mannose and Mannooligosaccharides to Concanavalin A

摘要: We report here, the first solution state evidence for role of water molecules in specific interaction carbohydrates with a legume lectin, concanavalin A. Concanavalin A from Canavalia ensiformis is protein containing 237 amino acid residues each monomer possessing one sugar binding site as well sites transition-metal ions, Mn2+ and Ca2+. The lectin binds specifically to α-anomers monosaccharides, d-glucopyranoside d-mannopyranoside, recognizes trimannosidic core N-linked glycoproteins, 3,6-di-O-(α-d-mannopyranosyl)-α-d-mannopyranoside high specificity, which constitutes minimum carbohydrate epitope that completely fills site. Sensitive isothermal titration microcalorimetry coupled osmotic stress strategy on was used dissect out differential involvement recognition branched trimannoside (3,6-di-O-(α-d-mannopyranosyl)-α-d-mannopyranoside), individual dimannosidic arms (3-O-(α...