Characterization of ecologically diverse viruses infecting co-occurring strains of cosmopolitan hyperhalophilic Bacteroidetes.

摘要: Hypersaline environments close to saturation harbor the highest density of virus-like particles reported for aquatic systems as well low microbial diversity. Thus, they offer unique settings studying virus-host interactions in nature. However, no viruses have been isolated so far infecting two most abundant inhabitants these (that is, euryarchaeon Haloquadratum walsbyi and bacteroidetes Salinibacter ruber). Here, using three different co-occurring strains, we eight ubiquitous S. ruber that constitute new genera (named 'Holosalinivirus', 'Kryptosalinivirus' 'Kairosalinivirus') according their genomic traits, host range, interaction capabilities abundances natural worldwide. Furthermore, get a more complete comprehensive view virus assemblages nature, microcosm experiment was set with mixture strains challenged brine concentrate, changes viral populations were monitored by metagenomics. Only closely related kairosalinivirus (strictly lytic wide range) enriched, despite initial abundance sample. Metagenomic analyses mesocosms allowed recovery genomes an ad hoc assembly strategy common metagenomic tools failed abundance, which underlines limitations current approaches. The increase this type accompanied diversity group, shown contig recruitment. These results are consistent scenario not only abundances, is key factor determining fate