Regulation of [ 3 H]D-Aspartate Release by the 5-F 2t -Isoprostane and Its 5-Epimer in Isolated Bovine Retina

摘要: We have evidence that 15-F2-isoprostanes (15-F2-IsoPs) regulate excitatory neurotransmitter release in ocular tissues. Although 5-F2-IsoPs are abundantly produced mammals, their pharmacological actions on remain unknown. In the present study, we compared effect of 5-F2-IsoP epimer pair, 5-F2t-IsoP (C5–OH β-position) and 5-epi-5-F2t-IsoP α-position), K+-evoked [3H]D-aspartate isolated bovine retina. further examined role prostanoid receptors inhibitory action overflow. Isolated retina were prepared for studies using superfusion method. (0.01 nM to 1 μM), attenuated a concentration-dependent manner, with 26.9% (P < 0.001; IC25 = 0.2 μM) being achieved at 1 μM concentration. Its 5-(S)-OH-epimer, (0.1 nM–1 μM), exhibited an biphasic action, yielding maximal response 35.7% (P < 0.001) 10 concentration drug (IC25 value 3 nM). prostanoid-receptor antagonists, AH 6809 (10 μM; EP1–3/DP) BAY-u3405 DP/Tx) no (10 nM–1 μM)-mediated inhibition, SC-19220 (1 μM; EP1) completely reversed (0.1 μM 1 μM)-induced attenuation release. Similarly, both SC-51322 23848 EP4) elicited by (0.1 μM) conclude 5-epi-5-F2t-IsoP, attenuate K+-induced presumably via receptor dependent mechanisms. The trans-orientation allylic hydroxyl group position C5 accounts apparent