Establishment, characterization and viral susceptibility of two cell lines derived from leopard wrasse Macropharyngodon geoffroy.
作者： J. Ma , S. Sun , L. Zeng , Y. Lu
DOI: 10.1111/JFB.12193
关键词:
摘要: Two new fish cell lines were established from skin (LWSK) and fin (LWFN) of leopard wrasse Macropharyngodon geoffroy. These cells grew optimally at 25° C in Leibovitz-15 medium supplemented with 10% foetal bovine serum. Proliferation M. geoffroy remained serum dependent up to passage 16, cell-plating efficiency ranged 12 16%. Karyotypic analysis these 8 indicated that both diploid a peak chromosomal count 144. PCR amplification 16S mitochondrial DNA the subsequent confirmed indeed derived Results viral challenge assays revealed LWSK LWFN shared patterns susceptibility similar six viruses tested: highly permissive channel catfish virus, spring viremia carp virus snakehead rhabdovirus high-yield production ranging 10 7·18±0.17 8·37±0·16 TCID 50 ml ―1 (mean ± S.D.). newly would be useful attempts isolate study aquatic viruses, particularly aetiology green turtle fibropapilloma as is known one common cleaner sea turtles.
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