Effect of YOYO-1 on the mechanical properties of DNA.

摘要: YOYO-1 is a green fluorescent dye which widely used to image single DNA molecules in solution for biophysical studies. However, the question of whether intercalation affects mechanical properties still not clearly answered. Investigators have put forth contradicting data on changes persistence length DNA. Here, we use atomic force microscopy systematically study due YOYO-1. We first measured length, contour and bending angle distribution DNA–YOYO-1 complex. find that remains unaffected with However increases linearly about 38% increase at full saturation 1 per 4 base pairs Next change topology relaxed closed circular after introduces supercoiling Our observations indicate results underwinding duplex, but does significantly length.