Genome-Based Discovery of a Novel Membrane-Bound 1,6-Dihydroxyphenazine Prenyltransferase from a Marine Actinomycete

摘要: Recently, novel prenylated derivatives of 1,6-dihydroxyphenazine have been isolated from the marine sponge-associated Streptomyces sp. SpC080624SC-11. Genome sequencing this strain now revealed a gene cluster containing all genes necessary for synthesis phenazine and isoprenoid moieties. Unexpectedly, however, did not contain with similarity to previously investigated prenyltransferases, but instead modest membrane-bound prenyltransferases ubiquinone menaquinone biosynthesis. Expression in E. coli isolation membrane fraction proved that encoded enzyme, Mpz10, catalyzes two successive prenylations 1,6-dihydroxyphenazine. Mpz10 is first example enzyme catalyzing prenylation substrate, one few examples enzymes involved aromatic secondary metabolites microorganisms.