Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing.

摘要: This study comprises the first attempt to describe planktonic bacterial communities of lakes from Byers Peninsula, one most significant limnological districts in Maritime Antarctica, leveraging next-generation sequencing (NGS) technologies. For survey, we selected 7 covering environmental gradient inland coastal lakes, some them sampled both surface and deep waters. Analysis provided just over 85,000 high quality sequences that were clustered into 864 unique Zero-radius Operational Taxonomic Units (ZOTUs) (i.e., 100% sequence similarity). Yet, several taxonomic uncertainties remained analysis likely suggesting occurrence local adaptations. The survey showed dominance phyla Proteobacteria Bacteroidetes. Among former, Gammaproteobacteria class, more specifically order Betaproteobacteriales, was dominant group, which seems be a common trend nutrient-limited Antarctic lakes. Most families genera ubiquitously detected belonging this class are indeed typical ultra-oligotrophic environments, commonly described as diazotrophs. On other hand, among members phylum Bacteroidetes, such Flavobacterium abundant shallowest thus demonstrating also benthic sediment-associated bacteria contributed water assemblages. Ordination analyses sorted assemblages mainly based on gradients nutrient availability conductivity i.e., salinity. However, transient associations, included groups Clostridiaceae Chloroflexi, occurred being forced by drivers influence nearby fauna airborne microorganisms. As intended, our NGS-based approach has much greater resolution compared previous studies conducted area confirmed large extent previously obtained patterns, reinforcing view hotspot microbial biodiversity within Antarctica. diversity allows use these aquatic ecosystems their sentinels for monitoring adaptive responses climate change rapidly warming area.