Development of Improved High-Performance Liquid Chromatography Conditions for Nonisotopic Detection of Isoaspartic Acid to Determine the Extent of Protein Deamidation
作者： A.D. Carlson , R.M. Riggin
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摘要: A rapid and accurate ion-pairing reversed-phase high-performance liquid chromatography (IP-RP-HPLC) procedure has been developed for nonisotopic detection of isoaspartic acid residues in protein or peptides resulting from deamidation asparagine residues. The IP-RP-HPLC specifically detects quantifies S-adenosylhomocysteine (SAH). SAH is a by-product the reaction between isoaspartyl methyltransferase (PIMT), S-adenosylmethionine (SAM), HPLC conditions described this paper have demonstrated to offer significantly better reproducibility compared earlier studies. method allows determination extent without use radioisotopes therefore offers significant advantages biopharmaceutical development laboratories.
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