Retinylamine Benefits Early Diabetic Retinopathy in Mice.

摘要: Recent evidence suggests an important role for outer retinal cells in the pathogenesis of diabetic retinopathy (DR). Here we investigated effect visual cycle inhibitor retinylamine (Ret-NH2) on development early DR lesions. Wild-type (WT) C57BL/6J mice (male, 2 months old when diabetes was induced) were made with streptozotocin, and some given Ret-NH2 once per week. Lecithin-retinol acyltransferase (LRAT)-deficient P23H mutant similarly studied. Mice euthanized after (WT Lrat−/−) 8 study to assess vascular histopathology, accumulation albumin, function, biochemical physiological abnormalities retina. Non-retinal effects examined leukocytes treated vivo. Superoxide generation expression inflammatory proteins significantly increased retinas or months, number degenerate capillaries albumin neural retina compared nondiabetic controls. Administration week inhibited capillary degeneration retina, reducing diabetes-induced superoxide proteins. also suppressed Lrat−/− mice. Leukocytes isolated from caused less cytotoxicity endothelial ex vivo than did control diabetics. lesions characteristic The constitutes a novel target inhibition DR.