Modeling continuum of epithelial mesenchymal transition plasticity

摘要: Living systems respond to ambient pathophysiological changes by altering their phenotype, a phenomenon called ‘phenotypic plasticity’. This program contains information about adaptive biological dynamism. Epithelial–mesenchymal transition (EMT) is one such process found be crucial in development, wound healing, and cancer wherein the epithelial cells with restricted migratory potential develop motile functions acquiring mesenchymal characteristics. In present study, phase contrast microscopy images of EMT induced HaCaT were acquired at 24 h intervals for 96 h. The expression study relevant pivotal molecules viz. F-actin, vimentin, fibronectin N-cadherin was carried out confirm process. Cells intuitively categorized into five distinct morphological phenotypes. A population 500 each temporal point selected quantify frequency occurrence. plastic interplay cell phenotypes from observations described as Markovian model formulated empirically using simple linear algebra, depict possible mechanisms cellular transformation among work employed qualitative, semi-quantitative quantitative tools towards illustration establishment continuum. Thus, it provides newer perspective understand embedded plasticity across spectrum.