Postmitotic death is the fate of constitutively proliferating cells in the subependymal layer of the adult mouse brain

摘要: The early development of the mammalian forebrain involves massive proliferation ventricular zone cells lining lateral ventricles. A remnant this highly proliferative region persists into adult life, where it is known as subependymal layer. We examined kinetics and fates mitotically active in subependyma mouse. medial edge, dorsolateral corner layer rostral portion ventricle each contained cells, but had highest percentage bromodeoxyuridine (BrdU)-labeled per unit area. Repeated injections BrdU over 14 hr revealed a curve for population with growth fraction 33%, indicating that 33% make up proliferating population. total cell cycle time was approximately 12.7 hr, an S-phase 4.2 hr. To examine fate these we injected low concentrations replication-deficient, recombinant retrovirus directly ventricles mice uptake by cells. Regardless survival postinjection (10 1 d, 2 or 8 d), number retrovirally labeled clone remained same (1 cells/clone). This suggests one progeny from division dies. Moreover, clones confined to were not seen surrounding brain tissue. Thus, while continue proliferate postmitotic death.