Activation of Cholera Toxin and E. Coli Heat-Labile Enterotoxin (LT) by Arf

作者: G. Pacheco-Rodriguez , Naoko Morinaga , Masatoshi Noda , J. Moss , M. Vaughan

DOI: 10.1007/1-4020-2593-9_10

关键词:

摘要: Cholera (CT) and heat labile E. coli (LT) enterotoxins are members of the AB5 family bacterial toxins that contain one catalytically active A subunit associated with a complex five B subunits. The subunits CT LT bind receptor, ganglioside GM1, at cell surface as required first step in entry holotoxin into target cells. is processed catalytic A1 linker A2 segments. holotoxin-receptor internalized transported to endoplasmic reticulum before release protein cytosol. ADP-ribosyltransferase activity critical determinant toxin potency. When bound GTP, all Arfs allosteric activators vitro. importance such interactions intoxication cells is, however, less clear. Recent reports suggest possibilities for clinically effective prevention action by interference initial binding GM1 receptor or inhibition CTA1 intestinal mucosa

参考文章(49)
J. Moss, S.J. Stanley, J.E. Morin, J.E. Dixon, Activation of choleragen by thiol: protein disulfide oxidoreductase. Journal of Biological Chemistry. ,vol. 255, pp. 11085- 11087 ,(1980) , 10.1016/S0021-9258(19)70255-5
S C Tsai, M Noda, R Adamik, P P Chang, H C Chen, J Moss, M Vaughan, Stimulation of choleragen enzymatic activities by GTP and two soluble proteins purified from bovine brain. Journal of Biological Chemistry. ,vol. 263, pp. 1768- 1772 ,(1988) , 10.1016/S0021-9258(19)77942-3
J. Moss, S.J. Stanley, M.C. Lin, NAD glycohydrolase and ADP-ribosyltransferase activities are intrinsic to the A1 peptide of choleragen. Journal of Biological Chemistry. ,vol. 254, pp. 11993- 11999 ,(1979) , 10.1016/S0021-9258(19)86416-5
Joel Moss, Stephen H. Richardson, Activation of Adenylate Cyclase by Heat-Labile Escherichia Coli Enterotoxin Journal of Clinical Investigation. ,vol. 62, pp. 281- 285 ,(1978) , 10.1172/JCI109127
R A Kahn, A G Gilman, The protein cofactor necessary for ADP-ribosylation of Gs by cholera toxin is itself a GTP binding protein. Journal of Biological Chemistry. ,vol. 261, pp. 7906- 7911 ,(1986) , 10.1016/S0021-9258(19)57489-0
P.A. Orlandi, P.K. Curran, P.H. Fishman, Brefeldin A blocks the response of cultured cells to cholera toxin. Implications for intracellular trafficking in toxin action. Journal of Biological Chemistry. ,vol. 268, pp. 12010- 12016 ,(1993) , 10.1016/S0021-9258(19)50301-5