Development of broadly targeted human endogenous gammaretroviral pol-based real time PCRs Quantitation of RNA expression in human tissues.
作者： Anna Forsman , Zhihong Yun , Lijuan Hu , Dmitrijs Uzhameckis , Patric Jern
DOI: 10.1016/J.JVIROMET.2005.04.016
关键词:
摘要: Endogenous retroviral sequences (ERVs) are dynamic genomic components with profound influences on gene expression and structure. Their extent of is not well known. Several broadly targeted real-time reverse transcription PCR (QPCRs) systems for surveillance RNA the major groups human gammaretroviral ERVs were constructed. The highly conserved transcriptase (RT) integrase (IN) domains pol used as targets PCRs, which both probe-based (TaqMan) probe-less (SYBR Green). Different levels primer probe degeneracy, or without inosine, tested. PCRs had sensitivities a few HERV nucleic acid copies per reaction. Specificities approximately expected from fit primers probes. Gammaretroviral was studied in different tissues. Each group specific pattern expression. HERV-E expressed testis, HERV-I/T brain HERV-H while HERV-W placenta. detected plasma 50 blood donors saliva 20 persons. In conclusion, set tools investigation created.
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