Periovulatory Expression of Hyaluronan and Proteoglycan Link Protein 1 (Hapln1) in the Rat Ovary: Hormonal Regulation and Potential Function

摘要: Periovulatory follicular matrix plays an important role in cumulus-oocyte complex (COC) expansion, ovulation, and luteal formation. Hyaluronan proteoglycan link protein 1 (HAPLN1), a component of matrix, was shown to enhance COC expansion vitro. However, the regulatory mechanisms periovulatory expression Hapln1 its granulosa cells have not been elucidated. We first determined pattern using pregnant mare serum gonadotropin/human chorionic gonadotropin (PMSG/hCG)-primed immature rat ovaries. transiently induced both intact ovaries at 8 h 12 after hCG injection. This vivo recapitulated by culturing preovulatory with hCG. The stimulatory effect blocked inhibition kinase A, phosphatidylinositol-dependent kinase, p38 MAPK, epidermal growth factor signaling, prostaglandin synthesis, revealing key mediators involved LH-induced expression. In addition, knockdown Runx1 Runx2 small interfering RNA or RUNX activities dominant-negative decreased agonist-induced Chromatin immunoprecipitation assays verified binding RUNX1 RUNX2 promoter cells. Luciferase reporter revealed that mutation sites completely obliterated activity promoter. These data conclusively identified proteins as crucial transcription regulators for Functionally, treatment HAPLN1 increased viability cultured number undergoing apoptosis, whereas viability. novel finding indicates may promote cell survival, which would facilitate their differentiation into