The genetic potential of N2 emission via denitrification and ANAMMOX from the soils and sediments of a created riverine treatment wetland complex

摘要: Abstract The biological nitrogen cycle is complex, and the origin of different compounds factors driving transformation processes in environments are not completely understood as new bacterial pathways genes associated with metabolism discovered. In this study, impact water regime, soil type chemical parameters on genetic potential nitrous oxide reduction anaerobic ammonium oxidation (ANAMMOX) soils sediments a created riverine wetland complex was determined by quantifying proportions reductase encoding nosZ gene clades I II, ANAMMOX-specific 16S rRNA gene, respectively. community structure profiled using high-throughput sequencing gene. results study showed that abundances were higher organic rich permanently flooded areas freshwater marshes. clade II (0.56–4.59% 0.48–3.01%, respectively) quite equally represented microbial communities studied soils. Both dependent hydrological conditions at site, showing values occasionally than sites two marshes area. proportion related to NH4–N, C/N, P, K, Ca concentrations soil, while relationship Mg content. clades’ positively correlated nirK community, negative detected between nirS genes. 0.00005% 0.084% sediments. Significantly ANAMMOX inflow An analysis fragments identified members three genera (Candidatus Brocadia, Candidatus Scalindua, Kuenenia) from indicated lower N2 emission via denitrification addition, current revealed can be both producing soils, since strong positive correlation detected.