Osteopetrosis in the toothless rat: failure of osteoclast differentiation and function.

作者: Popoff Sn , Osier Lk , Marks Sc

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摘要: Toothless is an osteopetrotic mutation in the rat characterized by reduced bone resorption, few osteoclasts and failure to be cured marrow transplants from normal littermates. The latter could due production of that cannot recruit or activate their precursors. We tested this hypothesis using a metatarsal organ culture system which metatarsals are cultured with various tissues act as sources osteoclast Mutant alone mutant tissue had numerous large mononuclear cells, osteoclasts, no spaces evidence resorption. Culture liver spleen caused changes number Cultures resorption spaces. Histochemical analyses demonstrated strong tartrate-resistant acid phosphatase (TRAP) staining was present all some cells metatarsals. before after contained TRAP-positive occasional, weakly cell only when co-cultured cells. interpret these data mean skeletal environment unable support development differentiation osteoclasts. These observations consistent toothless rats

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