Chapter 1. Methods to study no-go mRNA decay in Saccharomyces cerevisiae.

作者: Meenakshi K. Doma

DOI: 10.1016/S0076-6879(08)02401-4

关键词:

摘要: In eukaryotic cells, conserved mRNA surveillance systems target and degrade aberrant mRNAs, eliminating translation errors that occur during protein synthesis thereby imposing quality control of gene expression. Two such cytoplasmic systems, nonsense-mediated decay nonstop decay, have evolved to mRNAs with aberrancies in translation. A third novel system has been identified for yeast defects elongation due strong pause sites. This subset ribosome sites is recognized targeted degradation by an endonucleolytic cleavage a process referred as no-go (NGD). The methods described herein are designed aid the study NGD Saccharomyces cerevisiae. They include procedures create efficient pause, assay characteristics substrates, characterize NGD-dependent mRNA. logic design can be modulated used identification analysis RNA pathways other organisms.

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