A Synthetic Virus-Like Particle Streptococcal Vaccine Candidate Using B-Cell Epitopes from the Proline-Rich Region of Pneumococcal Surface Protein A.

摘要: Alternatives to the well-established capsular polysaccharide-based vaccines against Streptococcus pneumoniae that circumvent limitations arising from limited serotype coverage and emergence of resistance due capsule switching (serotype replacement) are being widely pursued. Much attention is now focused on development recombinant subunit based highly conserved pneumococcal surface proteins virulence factors. A further step might involve focusing host humoral immune response onto protective protein epitopes using as immunogens structurally optimized epitope mimetics. One approach deliver such mimetics system through use synthetic virus-like particles (SVLPs). SVLPs made coiled-coil lipopeptides designed spontaneously self-assemble into 20-30 nm diameter nanoparticles in aqueous buffer. Multivalent display generates immunogenic elicit strong epitope-specific responses without need for external adjuvants. Here, we set out demonstrate this can yield vaccine candidates able a response, derived proline-rich region (PspA). These streptococcal SVLP-based shown mice. Following active immunization challenge with lethal doses streptococcus, significant protection Furthermore, mimetic-specific monoclonal antibody mediate partial upon passive immunization. The results show combined may have potential an effective pneumoniae.