Enhancement of recombinant BmK AngM1 production in Pichia pastoris by regulating gene dosage, co-expressing with chaperones and fermenting in fed-batch mode.
作者： Qing-Hua Wang , Lan Liang , Wan-Cang Liu , Ting Gong , Jing-Jing Chen
DOI: 10.1080/10286020.2017.1311872
关键词:
摘要: The scorpion peptide BmK AngM1 was reported to exhibit evident analgesic effect, but its yield by extraction from venom limits the research and application. heterologous expression of achieved in Pichia pastoris our previous study. In order realize high-level recombinant (rBmK AngM1), gene dosage optimized engineered strains. rBmK four-copy strain reached up 100 mg/L, which further enhanced 190 mg/L co-expressing with chaperones PDI, BiP, HAC1. Moreover, 1200 mg/L high-density fermentation 10 L fermenter. Finally, 360 mg purified 1 L cultures a two-step purification method. efficient convenient techniques presented this study could facilitate scale-up for industrial production AngM1.
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