A self-contained system for the field production of plant recombinant interleukin-10

摘要: The production of pharmaceutical proteins in plants is creating a broad spectrum new high-value traits traditional crop species. As the these recombinant moves from bench to field scale, containment and presence unwanted secondary metabolites are significant practical issues. We have developed hybrid male-sterile low-alkaloid tobacco (MSLA) platform. Recombinant protein produced leaves that harvested prior flowering. If considered for direct vivo mammalian use background genotype addresses concerns about nicotine, male sterility further reduces risk gene leakage. applied this system human interleukin-10 (phIL-10), contra-inflammatory cytokine with potential application treatment inflammatory bowel disease autoimmune diseases. Transgenic lines properly assembled biologically active phIL-10 homodimer. Hybrids made by crossing single homozygous high-expressing line MSLA female were tested high density after 30 days. yields found be similar hybrids control. practical, self-contained plant proteins.