β-Glucuronidase activity during development of the male gametophyte from transgenic and non-transgenic plants
作者： Leon Plegt , Raoul J. Bino
DOI: 10.1007/BF00334371
关键词:
摘要: Beta-glucuronidase (GUS) was histochemically analyzed in anthers and pollen of potato, tobacco tomato. GUS activity determined transgenic plants containing a chimaeric gene untransformed plants. In at premeiotic meiotic stages sporogenous development, indigogenic precipitation indicative consistently manifest cells the vascular cylinder, connectivum stomium while no found tapetal tissues. At similar stages, anther sections did not show any indigo blue staining. later microspore both demonstrated high levels sporogenic cells. plants, also present stomium. These results indicate that tomato product localized tissue specifically. They an endogenous expressed temporal- spatial-specific manner tapetum transformed
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