Isobemisiose: an unusual trisaccharide abundant in the silverleaf whitefly, Bemisia argentifolii.

摘要: Abstract The major soluble carbohydrates in the silverleaf whitefly, Bemisia argentifolii, were glucose, α,α-trehalose and an unknown sugar. Analysis of sugar its chemical enzymatic digestion products by high-performance liquid chromatography (HPLC) showed that it was probably a trisaccharide, consisting entirely containing both isomaltose moieties. Matrix-assisted laser desorption mass spectrometry, spectrometry 13 C 1 H nuclear magnetic resonance spectroscopy confirmed trisaccharide with following structure: O-α- d -glucopyranosyl-(1→6)-O-α- -glucopyranosyl-(1↔1)-α- -glucopyranoside. This found primarily bodies B. argentifolii not their honeydew, is structurally similar to bemisiose [O-α- -glucopyranosyl-(1→4)-O-α- -glucopyranoside], first identified honeydew. Consequently, common name isobemisiose proposed for newly Isobemisiose, which has been previously reported occur nature, constituted as much 46% (w/w) ethanol-soluble sugars adult equivalent approximately 10% dry weight. It also quantities immature argentifolii. Isobemisiose detected two other whitefly species several aphids, but at lesser concentrations than Labeling pulse-chase experiments using [ 14 ]sucrose supplied artificial diet revealed label accumulated chased from more slowly either glucose or trehalose. Incubation cell-free extracts demonstrated these whiteflies contained necessary complement enzymes fully degrade glucose. These labeling indicate isobemisose storage carbohydrate