Differential Evolutionary Wiring of the Tyrosine Kinase Btk

摘要: Background A central question within biology is how intracellular signaling pathways are maintained throughout evolution. Btk29A considered to be the fly-homolog of mammalian Bruton’s tyrosine kinase (Btk), which a non-receptor tyrosine-kinase Tec-family. In cells, there single transcript splice-form and corresponding Btk-protein plays an important role for B-lymphocyte development with alterations human BTK gene causing immunodeficiency disease X-linked agammaglobulinemia in man related disorder mice. contrast, Drosophila locus encodes two splice-variants, where type 2-form more Btk product displaying than 80% homology. Drosophila, displays dynamic pattern expression through embryonic adult stages. Complete loss-of-function both splice-forms lethal, whereas selective absence reduces lifespan fly causes developmental abnormalities male genitalia. Methodology/Principal Findings Out 7004–7979 transcripts expressed four sample groups, 5587 (70–79%) were found all tissues strains. Here, we investigated 2 on transcriptomic level larval CNS heads. We used samples either selectively defective (Btk29AficP) or revertant flies restored 2-function (Btk29A(fic Exc1–16)). The whole profile different groups revealed Gene Ontology patterns reflecting head neuronal tissue, but not larvae. Conclusions In 2-deficient strains was no significant overlap between heads larvae respectively. Moreover, changes mammals, suggesting that evolutionary conservation confined components proximal signaling, corresponding, downstream transcriptional regulation has been differentially wired.