The HID EVOlution System for Automation of DNA Quantification and Short Tandem Repeat Analysis
作者： Rixun Fang , Jason Yingjie Liu , Heidi L Kijenski , Jacki Benfield , Ada Wong
DOI: 10.1016/J.JALA.2009.04.006
关键词:
摘要: The HID EVOlution—qPCR/STR Setup System enables automation of DNA quantitative real-time polymerase chain reaction (PCR) setup, normalization sample, and PCR setup for short tandem repeat (STR) analysis. EVOlution tracks sample reagent information facilitates data transfer quantification, normalization, STR analysis steps, eliminating the need manual processing repetitive entry. Instruments automated system include a Tecan Freedom EVO 150 robot liquid handling, 7500 Real-Time GeneAmp 9700 amplification, 3130xl Genetic Analyzer detection amplified fragments. Validation studies including reproducibility, accuracy, correlation, contamination were performed. Results demonstrated clean liquid-handling capabilities maintenance integrity. Variation in average allele peak height obtained using protocol was similar t...
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