Surface Material, Temperature, and Soil Effects on the Survival of Selected Foodborne Pathogens in the Presence of Condensate

摘要: The effects of surface type (stainless steel, acetal resin, and fiberglass reinforced plastic wall paneling [FRP]), soil, temperature on the survival Listeria monocytogenes, Salmonella spp., Yersinia enterocolitica, in presence condensate were evaluated. Surface coupons--half soiled with sterile porcine serum--were exposed to cell suspensions made from individual five-strain cocktails composed organisms same genus (10(7) CFU/ml) Butterfield's phosphate buffer incubated for 2 h at 25 degrees C allowing attachment cells coupon surfaces. Coupons rinsed remove unattached cells, either 4 or 10 under condensate-forming conditions, sampled six time intervals over a 15-day period. For enumeration, removed coupons by vigorous shaking 100 ml 3 g glass beads plated tryptic soy agar 0.6% yeast extract. Stainless steel did not support as well resin FRP. Acetal stainless less supportive than All surfaces supported trial equally. Temperature had little effect all across one exception. However, displayed growth FRP C. but death Serum protective L. monocytogenes surfaces, populations sustained significantly (P < = 0.05) higher numbers unsoiled coupons. didnot