Effect of 2-arachidonoylglycerol on myosin light chain phosphorylation and platelet activation: The role of phosphatidylinositol 3 kinase/AKT pathway.
作者： Maria Grazia Signorello , Giuliana Leoncini
DOI: 10.1016/J.BIOCHI.2014.07.014
关键词:
摘要: The endocannabinoid 2-arachidonoylglycerol (2-AG) can be considered a true agonist as it is able to activate human platelets stimulating arachidonic acid release, thromboxane B2 formation and calcium intracellular elevation. Recently we have shown that 2-AG induces rapid myosin light chain (MLC) phosphorylation/activation, early mediated by RhoA kinase (ROCK) signalling pathway later kinase. aim of the present study was investigate role phosphatidylinositol 3 (PI3K)/AKT in MLC phosphorylation some downstream events such actin polymerization, ATP secretion aggregation. We demonstrated PI3K particular isoforms α β AKT phosphorylation. stimulation PI3K/AKT activates ROCK. ROCK directly involved phase activation thr18 strengthened through phosphatase inhibition, accomplished MYPT1, catalytic subunit phosphatase, overall In addition found PI3Kα/β are mechanisms leading aggregation stimulated 2-AG.
core.ac.uk
sci-hub.se 参考文章(48)
Anthony Baurand, Anita Eckly, Nadège Bari, Catherine Léon, Béatrice Hechler, Jean-Pierre Cazenave, Christian Gachet, None, Desensitization of the platelet aggregation response to ADP: differential down-regulation of the P2Y1 and P2cyc receptors. Thrombosis and Haemostasis. ,vol. 84, pp. 484- 491 ,(2000) , 10.1055/S-0037-1614049
T. M. GETZ, C. A. DANGELMAIER, J. JIN, J. L. DANIEL, S. P. KUNAPULI, Differential phosphorylation of myosin light chain (Thr)18 and (Ser)19 and functional implications in platelets. Journal of Thrombosis and Haemostasis. ,vol. 8, pp. 2283- 2293 ,(2010) , 10.1111/J.1538-7836.2010.04000.X
J L Daniel, I R Molish, M Rigmaiden, G Stewart, Evidence for a role of myosin phosphorylation in the initiation of the platelet shape change response. Journal of Biological Chemistry. ,vol. 259, pp. 9826- 9831 ,(1984) , 10.1016/S0021-9258(17)42774-8
Yoshinori Suzuki, Masatoshi Yamamoto, Hideo Wada, Masaaki Ito, Takeshi Nakano, Yasuharu Sasaki, Shuh Narumiya, Hiroshi Shiku, Masakatsu Nishikawa, Agonist-induced regulation of myosin phosphatase activity in human platelets through activation of Rho-kinase Blood. ,vol. 93, pp. 3408- 3417 ,(1999) , 10.1182/BLOOD.V93.10.3408.410K37_3408_3417
M Ikebe, R Ikebe, H Kamisoyama, S Reardon, J P Schwonek, C R Sanders, M Matsuura, Function of the NH2-terminal domain of the regulatory light chain on the regulation of smooth muscle myosin. Journal of Biological Chemistry. ,vol. 269, pp. 28173- 28180 ,(1994) , 10.1016/S0021-9258(18)46910-4
Paola E. J. van der Meijden, Simone M. Schoenwaelder, Marion A. H. Feijge, Judith M. E. M. Cosemans, Imke C. A. Munnix, Reinhard Wetzker, Regine Heller, Shaun P. Jackson, Johan W. M. Heemskerk, Dual P2Y12 receptor signaling in thrombin‐stimulated platelets – involvement of phosphoinositide 3‐kinase β but not γ isoform in Ca2+ mobilization and procoagulant activity FEBS Journal. ,vol. 275, pp. 371- 385 ,(2008) , 10.1111/J.1742-4658.2007.06207.X
J E Fox, D R Phillips, Role of phosphorylation in mediating the association of myosin with the cytoskeletal structures of human platelets. Journal of Biological Chemistry. ,vol. 257, pp. 4120- 4126 ,(1982) , 10.1016/S0021-9258(18)34694-5
Mauro Maccarrone, Monica Bari, Adriana Menichelli, Elena Giuliani, Domenico Del Principe, Alessandro Finazzi-Agrò, Human platelets bind and degrade 2-arachidonoylglycerol, which activates these cells through a cannabinoid receptor. FEBS Journal. ,vol. 268, pp. 819- 825 ,(2001) , 10.1046/J.1432-1327.2001.01942.X
Maria Grazia Signorello, Enrica Giacobbe, Mario Passalacqua, Giuliana Leoncini, The 2-arachidonoylglycerol effect on myosin light chain phosphorylation in human platelets. Biochimie. ,vol. 95, pp. 1620- 1628 ,(2013) , 10.1016/J.BIOCHI.2013.05.003
Masakatsu Nishikawa, Toshio Tanaka, Hiroyoshi Hidaka, Ca2+-calmodulin-dependent phosphorylation and platelet secretion. Nature. ,vol. 287, pp. 863- 865 ,(1980) , 10.1038/287863A0