Methods of using oligonucleotides comprising a molecular switch
作者： Bob D. Brown , Lyle J. Arnold
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摘要: This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The portion of the probe is particularly sensitive identity sequences complementary switch. Oligonucleotides containing are applicable all kinds hybridization processes. Due sensitivity domain oligonucleotide, probes useful identification single point mismatches. More specifically, portion, but not all, oligonucleotide becomes unbound from mismatched target. further methods using said for research reagents, and clinical diagnostics. An exemplary comprises first hybridizable domain, second bridging block third binding domain.
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