Osteopontin inhibition of miR-129-3p enhances IL-17 expression and monocyte migration in rheumatoid arthritis.

摘要: Abstract Background Osteopontin (OPN) is an important proinflammatory cytokine in rheumatoid arthritis (RA). Levels of OPN have been shown to be significantly correlated with interleukin-17 (IL-17) production and expression Th17 cells the synovial fluid RA patients. Here, we investigated role monocyte migration, IL-17 osteoblasts. Methods profiles osteoarthritis (OA) were determined by enzyme-linked immunosorbent assay (ELISA). The microRNA, miR-129-3p, osteoblasts was analyzed real-time quantitative polymerase chain reaction (qPCR). Immunoreactive proteins spotted Western blotting. We used collagen-induced (CIA) mouse model investigate migration during RA. Results higher as compared OA samples. also found that promotes thereby enhances via Syk/PI3K/Akt signaling pathway. miR-129-3p negatively regulated signal cascade. In contrast, lentiviral vectors expressing short hairpin RNA inhibited ameliorated articular swelling, cartilage erosion infiltration ankle joints CIA mice. Conclusion To our knowledge, study first describe how upregulating disease. Significance These findings indicate could serve a potential therapeutic target for treatment