A simple adenosine fluorescent aptasensor based on the quenching ability of guanine

摘要: Based on guanine (G)-quenching, a simple and sensitive fluorescent aptasensor for the detection of adenosine was constructed. G located end DNA strand selected as fluorescence quencher FAM fluorophore. The FAM-labeled aptamer (FAM-DNA) partly complementary to sequence containing five end. In absence adenosine, hybridization between FAM-DNA cause approach FAM, hence, effectively quenched by photoinduced electron transfer (PET) G. presence reacts with starts structural switching, resulting in release recovery FAM. linear range present proposed sensor is 20–200 μM, limit down 6.7 μM (S/N = 3). Most importantly, no organic dye molecules-labeled or extra nanomaterials were used quenchers this strategy. method simple, inexpensive has potential application clinical diagnostic assays.