Low false response rates in screening a hundred veterinary drug residues in foodstuffs by LC-MS/MS with analyte-specific correction of the matrix effect

摘要: Abstract As a response to the demand for better efficiency and reliability in monitoring of veterinary drug residues food, liquid chromatography-mass spectrometry (LC-MS), either tandem with quadrupoles (LC-MS/MS) or high resolution systems (LC-HRMS), has become an attractive tool screening purposes. However, lack reproducibility ionization yield observed by electrospray remains major limitation having powerful LC-MS platforms able screen more than hundred substances broad range food on reliable manner. False responses are not scarce, require insightful approaches mitigate them. This study describes analyte-specific correction matrix effect (SACME), as strategy based upon comparison chromatographic signals between sample analyzed ‘as is’ replicate that preliminarily been spiked at action level. Emphasis was put level confidence results achieved SACME, providing self-quality control each tested. The significant decrease variability compared absolute abundance (SAA) is shown, perspective wide matrices (raw materials, processed ingredients finished products) without tedious technical precautions highlighted. In end, false rates compared, data demonstrate unambiguously benefit attenuation against common approach using areas. No positive (FP) obtained whilst minimum 2–14 compounds (over 105 scope method) exhibited SAA. With negative (FN) low 1 5 were obtained, remained 15–31