Natural genetic variability reduces recalcitrance in poplar.

摘要: Lignin content and structure are known to affect recalcitrance of lignocellulosic biomass chemical/biochemical conversion. Previously, we identified rare Populus trichocarpa natural variants with significantly reduced lignin content. Because may lower recalcitrance, 18 along 4 comparators, BESC standard was analyzed for composition structural carbohydrates lignin. Sugar yields from these plants were measured at 5 process conditions: one just enzymatic hydrolysis without pretreatment four via our combined high-throughput hot water co-hydrolysis (HTPH) technique. Mean glucan + xylan the best yield poplar 34 50 relative percent higher than high comparator (BESC-316) highest severity HTPH condition, respectively. The ability solubilize a large portion xylan solids led small differences in among variants. However, showed glucan yields, hence did not display lowest moderate compared variants, but on other hand, low consistent top performer all conditions. Furthermore, (GW-11012) 15 absolute increase most severe condition. Overall, variant rankings varied greatly severity, deconstruction enhanced when temperature increased 140 160 180 °C same factor. Glucan comparator. significant effect processing conditions performance rankings, selection performing biofuel feedstocks should be based sugar tested that represent industrial practice. From feedstock perspective, SKWE-24-2 GW-11012, provide key insights into genetic improvement versatile biofuels varieties.