Myeloma progenitors in the blood of patients with aggressive or minimal disease: engraftment and self-renewal of primary human myeloma in the bone marrow of NOD SCID mice.

摘要: The myelomagenic capacity of clonotypic myeloma cells in G-CSF mobilized blood was tested by xenotransplant. Intracardiac (IC) injection NOD SCID mice with peripheral from 5 patients who had aggressive led to lytic bone lesions, human Ig the serum, plasma cells, and a high frequency murine marrow (BM). Human B were detected BM, spleen, blood. Injection ex vivo multiple directly into sternal BM (intraosseus [IO]) leads femoral BM. This shows that has spread primary site distant locations. By using cellular limiting dilution PCR assay quantify lineage we confirmed homed after IC IO injection. progenitor undergoes self-renewal as demonstrated transfer secondary recipient mouse. For 6 7 patients, have minimal disease, taken at time mobilization or cryopreservation, included progenitors identified engraftment and/or disease mice. indicates are cyclophosphamide/G-CSF. Their ability generate xenotransplant model implies such also when reinfused suggests need for an effective strategy purge them before transplant.