Regulation of Nucleosome Architecture and Factor Binding Revealed by Nuclease Footprinting of the ESC Genome.
作者： Sarah J. Hainer , Thomas G. Fazzio
DOI: 10.1016/J.CELREP.2015.08.071
关键词:
摘要: Functional interactions between gene regulatory factors and chromatin architecture have been difficult to directly assess. Here, we use micrococcal nuclease (MNase) footprinting probe the functions of two chromatin-remodeling complexes. By simultaneously quantifying alterations in small MNase footprints over binding sites 30 mouse embryonic stem cells (ESCs), provide evidence that esBAF Mbd3/NuRD modulate several proteins. In addition, find nucleosome occupancy is reduced at specific loci favor subnucleosomes upon depletion esBAF, including histone H2A.Z localization. Consistent with these data, demonstrate required for normal localization ESCs, suggesting either stabilizes H2A.Z-containing nucleosomes or promotes subnucleosome conversion by facilitating deposition. Therefore, integrative examination reveals insights into dynamics functional structure key gene-regulatory factors.
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