NK Cell Tolerance to TLR Agonists Mediated by Regulatory T Cells after Polymicrobial Sepsis

摘要: As sensors of infection, innate immune cells are able to recognize pathogen-associated molecular patterns by receptors such as TLRs. NK present in many tissues contribute inflammatory processes, particularly through the production IFN-γ. They may display a protective role during infection but also detrimental sterile or infectious systemic response syndrome. Nevertheless, exact status bacterial sepsis and their capacity directly respond TLR agonists remain unclear. The expression TLRs has been widely studied analyzing mRNA these receptors. aim this study was gain insight into TLR2/TLR4/TLR9 on/in murine at protein level determine if were induce cytokine production. We show, flow cytometry, strong intracellular TLR2 low TLR4 freshly isolated spleen cells, similar that TLR9. In vitro, purified TLR2, TLR4, TLR9 agonists, synergy with activating cytokines (IL-2, IL-15, and/or IL-18), produce proinflammatory (IFN-γ GM-CSF). Finally, we explored possible tolerance after polymicrobial (experimental peritonitis). For first time, our knowledge, shown become tolerant terms sepsis. show is associated reduction CD27(+)CD11b(-) subset related presence regulatory T mainly mediated TGF-β.