DNA-binding by the glucocorticoid receptor: A structural and functional analysis

摘要: The glucocorticoid receptor belongs to a family of ligand activated nuclear receptors. This includes, in addition the receptors for steroid hormones, thyroid hormone, retinoic acid and 1,25-dihydroxy vitamin D3 as well some with yet unknown ligands. DNA-binding domain has been expressed E. coli. purified protein binds same DNA sequences native is therefore suitable biochemical structural studies function protein. shown bind dimer its site. Protein-protein interactions facilitate segment responsible these identified close C-terminal zinc-binding receptors, their related sites, provides an opportunity study determinants sequence recognition. A N-terminal zinc ion be target specificity estrogen domains include nine conserved cysteine residues which have coordinate two ions required integrity ability domain. motif recognition, based around ions, was first described transcription factor IIIA were believed recognize via similar motif. However, three-dimensional structure determination shows that clearly different from TFIIIA type domains.