Gadd45a gene silencing by RNAi promotes cell proliferation and inhibits apoptosis and senescence in skin squamous cell carcinoma through the p53 signaling pathway.

摘要: Skin squamous cell carcinoma (SCC) is generally considered as nonaggressive lesions and mainly caused by ultraviolet (UV) radiation. Gadd45a a key component protecting skin against UV-induced tumors. For that, the study aims to investigate mechanism of gene silencing on proliferation, apoptosis, senescence in nude mice with SCC through p53 signaling pathway. Healthy was collected normal group 40 mouse models were successfully established model group, which sub-divided into five groups. The incidence, size, weight tumor observed. mRNA expression Gadd45a, Cyclin B1, MMP-2, Bcl-2, Bax determined RT-qPCR. Cell viability, cycle detected MTT assay, flow cytometry, β-galactosidase staining, respectively. levels inflammatory factors vascular endothelial growth factor (VEGF) using ELISA. protein rate mutant immunohistochemistry. Mice transfected siGadd45a showed increased weight. Cells decrease Bax; increase p53, IL-1α, IL-1β, IL-6, TNF-α, VEGF. apoptosis inhibited, while viability proliferation promoted after treatment. results reveal that increases reduces pathway SCC.