Differential adaptive response to hyperosmolarity of 3T3 and transformed SV3T3 cells

摘要: Both 3T3 and simian virus 40-transformed (SV3T3) cells were used to investigate differences in population kinetics, protein synthesis, monovalent ion levels, amino acid accumulations between normal transformed exposed hyperosmolarity at 0.5 Osm. Under similar culture conditions, SV3T3 found be more sensitive their proliferative response than the hyperosmolar treatment. In cells, increase transport of acids was less sustained associated with higher levels accumulated acids. The equilibrium distribution intracellular cations rate synthesis also returned faster baseline values cells. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis revealed induction a 69-kDa polypeptide but not after exposure hyperosmolarity. On electrofocusing relative mass analysis, this closely migrated 70-kDa heat shock (hsp) family, although it unrelated immunologically inducible 72-kDa hsp.