Long-term field persistence of Beauveria brongniartii strains applied as biocontrol agents against European cockchafer larvae in Switzerland

摘要: In field tests between 1985 and 1992, various sites which were infested with Melolontha melolontha treated the biocontrol fungus Beauveria brongniartii. The was applied either as blastospores or commercially available colonized barley kernels (FCBK). 1998/1999, soil samples collected from test B. brongniartii isolates recovered maintained in a culture collection. Isolates this collection subjected to genetic analyses by use of specific microsatellite markers. strains detected at all up 14 years after their application. At some sites, populations consisted exclusively, whereas other indigenous that may have arisen mutations interactions among present addition strains. results suggested could coexist same habitat. Demonstration long-term persistence fields supported success approach for biological control M. melolontha.