Injectable Polypeptide Hydrogels with Tunable Microenvironment for 3D Spreading and Chondrogenic Differentiation of Bone-Marrow-Derived Mesenchymal Stem Cells.
作者： Kaixuan Ren , Haitao Cui , Qinghua Xu , Chaoliang He , Gao Li
DOI: 10.1021/ACS.BIOMAC.6B00884
关键词:
摘要: Bone-marrow-derived mesenchymal stem cells (BMSCs) possess vast potential for tissue engineering and regenerative medicine. In this study, an injectable hydrogel comprising poly(l-glutamic acid)-graft-tyramine (PLG-g-TA) with tunable microenvironment was developed via enzyme-catalyzed cross-linking used as artificial extracellular matrix (ECM) to explore the behaviors of BMSCs during three-dimensional (3D) culture. It found that mechanical property, porous structure well degradation process hydrogels could be tuned by changing copolymer concentration. The PLG-g-TA showed good cytocompatibility in vitro. After being subcutaneously injected into back rats, degraded gradually within 8 weeks exhibited biocompatibility vivo. were then encapsulated polypeptide-based different concentration investigate influence 3D on cell behaviors. is intriguing note that...
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