Interaction of cyclin-dependent kinase 2 and the Lyn tyrosine kinase in cells treated with 1-beta-D-arabinofuranosylcytosine.

摘要: The cyclin dependent kinase 2 (Cdk2) is required for initiation and progression of DNA replication. Activation Cdk2 involves binding to E or A dephosphorylation Tyr15. present studies demonstrate that treatment U-937 cells with 1-beta-D-arabinofuranosylcytosine (ara-C) associated tyrosine phosphorylation inhibition activity. results also directly associates the Src-like Lyn as a consequence ara-C-treatment. Confocal microscopy show detectable in nucleus it colocalises Cdk2. Subcellular fractionation coimmunoprecipitation further nuclear We phosphorylates Tyr15 incubation These findings suggest association ara-C-treated may contribute regulation Cdk2-dependent cell cycle checkpoints.