Local intestinal immune responses to infections with Trichinella spiralis. Real-time, continuous assay of cytokines in the intestinal (afferent) and efferent thoracic duct lymph of rats.
作者： Deborah Negrao-Correa , Robin Bell , Kalyanasundanam Ramaswamy
DOI:
关键词:
摘要: Levels of IL-4, IL-5, TNF-alpha, and IFN-gamma were quantitated in the intestinal (afferent) efferent thoracic duct lymph rats during course (0 to 289 h) an infection with Trichinella spiralis. Intestinal was collected by cannulating ducts mesenteric lymphadenectomized animals. These studies showed that cytokines typical a Th2 type (IL-4 IL-5) Th1 (IFN-gamma) simultaneously detected first 8 days after infection. Worm expulsion (day 11 12) associated increased levels IL-4 IL-5 lymph. rose as early 15 20 h remained elevated throughout activity appeared 60 reached peak worm expulsion. Despite predominantly nature cytokine response, several cycles high low production A comparison between values no significant differences or suggesting contribution node However, TNF-alpha lower compared consumption. Adoptive transfer experiments protective CD4+CD45RC- cells primed gut for more rapid TH2-type response faster than primary In contrast, adoptive CD4+CD45RC+ Th1-type(IFN-gamma) response. demonstrate novel method measuring real-time changes active
参考文章(12)
Grotz Mr, Ding J, Huang Q, Guo W, Deitch Ea, Comparison of plasma cytokine levels in rats subjected to superior mesenteric artery occlusion or hemorrhagic shock. Shock. ,vol. 3, pp. 362- ,(1995)
A Greenwood, C H Wang, R G Bell, M Korenaga, T-helper subset function in the gut of rats: differential stimulation of eosinophils, mucosal mast cells and antibody-forming cells by OX8- OX22- and OX8- OX22+ cells. Immunology. ,vol. 71, pp. 166- 175 ,(1990)
D Wakelin, R K Grencis, Short lived, dividing cells mediate adoptive transfer of immunity to Trichinella spiralis in mice. I. Availability of cells in primary and secondary infections in relation to cellular changes in the mesenteric lymph node. Immunology. ,vol. 46, pp. 443- 450 ,(1982)
A Svetić, KB Madden, XD Zhou, Pin Lu, IM Katona, FD Finkelman, JF Urban, WC Gause, None, A primary intestinal helminthic infection rapidly induces a gut-associated elevation of Th2-associated cytokines and IL-3. Journal of Immunology. ,vol. 150, pp. 3434- 3441 ,(1993)
Elizabeth D. Hay, Cell Biology of Extracellular Matrix ,(1988)
JF Urban, Ildy M Katona, William E Paul, Fred D Finkelman, None, Interleukin 4 is important in protective immunity to a gastrointestinal nematode infection in mice. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 5513- 5517 ,(1991) , 10.1073/PNAS.88.13.5513
Phillip Scott, Edward Pearce, Allen W. Cheever, Robert L. Coffman, Alan Sher, Role of cytokines and CD4+ T-cell subsets in the regulation of parasite immunity and disease Immunological Reviews. ,vol. 112, pp. 161- 182 ,(1989) , 10.1111/J.1600-065X.1989.TB00557.X
Jan Vilček, Igor C. Oliveira, Recent Progress in the Elucidation of Interferon-Gamma Actions: Molecular Biology and Biological Functions International Archives of Allergy and Immunology. ,vol. 104, pp. 311- 316 ,(1994) , 10.1159/000236685
R.G. Bell, D.D. McGregor, Trichinella spiralis: expression of rapid expulsion in rats exposed to an abbreviated enteral infection. Experimental Parasitology. ,vol. 48, pp. 42- 50 ,(1979) , 10.1016/0014-4894(79)90053-5
P. H. van der Meide, M. Dubbeld, K. Vijverberg, T. Kos, H. Schellekens, The Purification and Characterization of Rat Gamma Interferon by Use of Two Monoclonal Antibodies Journal of General Virology. ,vol. 67, pp. 1059- 1071 ,(1986) , 10.1099/0022-1317-67-6-1059