In vitro behavior of human mesenchymal stem cells on poly(N-isopropylacrylamide) based biointerfaces obtained by matrix assisted pulsed laser evaporation

摘要: Abstract The use of smart coatings with tunable characteristics in bioengineering fields is directly correlated the surface chemical and topographical properties, method preparation, also type cells implied for specific application. In this work, a versatile modification technique based on lasers (Matrix-Assisted Pulsed Laser Evaporation (MAPLE)) was used to yield poly(N-isopropylacrylamide) (pNIPAM) its derivatives (amine, azide amide terminated pNIPAM) functional termoresponsive thin films. Surface properties pNIPAM derivative films such as morphology, roughness hydrophobic/hydrophilic character, well thermoresponsive capacity were investigated by atomic force microscopy contact angle measurements. analysed Fourier Transform Infrared Spectroscopy (FTIR). functionality kept all samples obtained MAPLE thermoresponse demonstrated change thickness values when temperature shifted from 37 °C 24 °C materials tested, smaller maleimide pNIPAM. Biological assays performed vitro (fluorescence Scanning Electron Microscopy (SEM)) confirmed conditioning early mesenchymal stem cell (MSC) growth chemistry coatings. imaging analysis revealed no cytotoxic effect surfaces irrespective functionalization. An increased proliferation rate grown pNIPAM-azide lower density pNIPAM-maleimide compared observed, which can direct their potential regenerative medicine approaches.