Intranasal Delivery of Genistein-Loaded Nanoparticles as a Potential Preventive System against Neurodegenerative Disorders.

摘要: Genistein has been reported to have antioxidant and neuroprotective activity. Despite encouraging in vitro vivo results, several disadvantages such as poor water solubility, rapid metabolism, low oral bioavailability limit the clinical application of genistein. The aim this study was design characterize genistein-loaded chitosan nanoparticles for intranasal drug delivery, prepared by ionic gelation technique using sodium hexametaphosphate. Nanoparticles were characterized their cytotoxicity tested on PC12 cells. Genistein-loaded prepared, hexametaphosphate used a valid alternative well-known cross-linkers. Nanoparticle characteristics well physical stability affected formulation composition manufacturing. Small (mean diameters 200–300 nm) homogeneous obtained able improve genistein penetration through nasal mucosa compared pure dispersions showed pH consistent with fluid preserved cell vitality.