Image Analytic Techniques for Quantification of Immunohistochemical Staining in the Nervous System

摘要: Publisher Summary Image analysis is a set of techniques that permit the investigator to obtain quantitative information from morphological preparations. When used properly, image histological sections powerful tool capable providing meaningful data and sensitive subtle alterations in tissue staining characteristics. However, when improperly, can produce erroneous results. As are highly dependent on computer processing, many these errors be introduced inadvertently, with profound This chapter provides an baseline knowledge regarding analytic maximize amount resulting minimize magnitude error. The further assists scientist who has little or no previous experience methodologies understand essential steps should carried out immunohistochemically stained material rudiments theoretical basis for each steps. A number different hardware configurations have been employed analysis, including scanning densitometry, drum video-based densitometry. latter configuration now most widely reasons, flexibility cost. discusses only systems.