Method and apparatus for ligand detection

作者: Bernard Hoop , James A. Burton

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摘要: A process and apparatus has been developed for radioassay of ligand in solution which eliminates the separation step required conventional techniques. chamber is provided containing a quenching solution, plurality molecules receptor molecules. One pluralities forms free species labelled with beta particle emitter while other immobilized on solid support, e.g., wall or microbead, within chamber. Ligand introduced sample competes already sites allowed to diffuse about detector communication at fixed position detects only those particles emitted from distance solution. The properties are used place step. easily adapted continuous monitoring level particularly well suited use radioimmunoassays. can be miniturized allowing implantation an animal body vivo bodily fluids.