Disruption of glycosylphosphatidylinositol-anchored lipid transfer protein gene altered cuticular lipid composition, increased plastoglobules, and enhanced susceptibility to infection by the fungal pathogen Alternaria brassicicola.

摘要: All aerial parts of vascular plants are covered with cuticular waxes, which synthesized by extensive export intracellular lipids from epidermal cells to the surface. Although it has been suggested that plant lipid transfer proteins (LTPs) involved in transport, planta evidence is still not clear. In this study, a glycosylphosphatidylinositol-anchored LTP (LTPG1) showing higher expression peels stems than was identified an Arabidopsis (Arabidopsis thaliana) genome-wide microarray analysis. The LTPG1 observed various tissues, including epidermis, stem cortex, bundles, mesophyll cells, root tips, pollen, and early-developing seeds. found be localized plasma membrane. Disruption gene caused alterations composition, but no significant changes on total wax cutin monomer loads were seen. largest reduction (10 mass %) ltpg1 mutant C29 alkane, major component waxes siliques. reduced content overcome increases secondary alcohols ketone loads. ultrastructure analysis showed more diffuse layer structure, protrusions cytoplasm into vacuole increase plastoglobules cortex leaf cells. Furthermore, susceptible infection fungus Alternaria brassicicola wild type. Taken together, these results indicated contributed either directly or indirectly accumulation.