Nardostachys jatamansi Root Extract Modulates the Growth of IMR-32 and SK-N-MC Neuroblastoma Cell Lines Through MYCN Mediated Regulation of MDM2 and p53.
作者： Snehal Suryavanshi , Rashmi Deshpande , Ruchika Kaul-Ghanekar , Prerna Raina
关键词:
摘要: Aim: The present study evaluated the effect of ethanolic extract Nardostachys jatamansi roots (NJet) on MYCN mediated regulation expression MDM2 and p53 proteins in neuroblastoma cell lines, IMR-32 SK-N-MC. Materials Methods: NJet viability was determined by MTT; growth kinetics trypan blue dye exclusion method soft agar assay. p53, response to treatment immunoblotting. Results: decreased cells without affecting non-cancerous, HEK-293 cells. It altered cancer a dose-dependent manner. down regulated with simultaneous increase tumor suppressor protein p53. Conclusions: data demonstrated that SK-N-MC through reduction lead expression. These preliminary results warrant further depth studies explore therapeutic potential management neuroblastoma. Abbreviations used: NJet: N. jatamansie thanolic extract; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylthiazolium bromide; FBS: Fetal bovine serum; FITC: Fluorescein isothiocyanate
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