Specific method for determination of gefitinib in human plasma, mouse plasma and tissues using high performance liquid chromatography coupled to tandem mass spectrometry

摘要: A rapid, sensitive and specific method was developed validated using liquid chromatography-tandem mass spectrometry (LC/MS/MS) for determination of gefitinib in human plasma mouse tissue. Sample preparation involved a single protein precipitation step by the addition 0.1 mL or 200 mg/mL tissue homogenate diluted 1/10 with 0.3 acetonitrile. Separation compounds interest, including internal standard (d8)-gefitinib, achieved on Waters X-Terra C18 (50 mm x 2.1 i.d., 3.5 microm) analytical column mobile phase consisting acetonitrile-water (70:30, v/v) containing 0.1% formic acid isocratic flow at 0.15 mL/min 3 min. The analytes were monitored tandem electrospray positive ionization. Linear calibration curves generated over range 1-1000 ng/mL samples 5-1000 values coefficient > 0.99. both within- between-day precision accuracy well within generally accepted criteria methods (< 15%). This subsequently used to measure concentrations mice following administration dose 150 mg/kg intraperitoneally cancer patients receiving an oral daily 250 mg.