[Mathematical modelling of glycolysis and adenine nucleotide metabolism of human erythrocytes. I. Reaction-kinetic statements, analysis of in vivo state and determination of starting conditions for in vitro experiments].

摘要: A mathematical model of energy metabolism human red cells is presented, which includes besides the glycolytic reactions adenine nucleotide metabolism. The based on network chemical reactions, thermodynamic equilibrium constants fast reversible and kinetic equations for irreversible enzyme reactions. consists a system 16 differential allows evaluation metabolic levels in steady state corresponding to vivo erythrocytes with data enzymes derived from vitro experiments. dependence metabolites activity some analysed characterize regulatory properties system. comparison experimental makes it possible estimate values controversial parameters. Estimates parameters following intracellular processes are presented: 1) rate constant AMP-phosphatase, 2) maximum adenylate deaminase, 3) phosphoribosylpyrophosphate transferase 4) adenosine transport through cell membrane. simulation preparatory phase before incubation indicates, that also permits compute time course changes metabolites. To solve initial problem stiff equation integrated numerically by an efficient program without application quasi-steady-state approximation.